Resolvase

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Yeast Holliday junction resolvase dimer complex with sulfate, 1kcf

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Function

Resolvase or recombinase (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase.

Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands^[1].
Tyr-recombinases include Cre which recombines loxP sites, FLP and lambda integrase (LamInt).
Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.
RadA recombinase promotes DNA recombination. Detailed analysis of its dimeric structure suggests mechanisms for junction isomerization and communication between the two active sites ^[2].
RadB recombinase is a prologue of RadA assisting in RadA-mediated strand exchange ^[3].
Hin-recombinase (HRec) is a protein of Salmonella. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions^[4].

3D structures of resolvase

Resolvase 3D structures

↑ West SC. The search for a human Holliday junction resolvase. Biochem Soc Trans. 2009 Jun;37(Pt 3):519-26. doi: 10.1042/BST0370519. PMID:19442245 doi:https://dx.doi.org/10.1042/BST0370519
↑ Haldenby S, White MF, Allers T. RecA family proteins in archaea: RadA and its cousins. Biochem Soc Trans. 2009 Feb;37(Pt 1):102-7. doi: 10.1042/BST0370102. PMID:19143611 doi:https://dx.doi.org/10.1042/BST0370102
↑ Wardell K, Haldenby S, Jones N, Liddell S, Ngo GHP, Allers T. RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator. DNA Repair (Amst). 2017 Jul;55:7-16. PMID:28501701 doi:10.1016/j.dnarep.2017.04.005
↑ Dhar G, Sanders ER, Johnson RC. Architecture of the hin synaptic complex during recombination: the recombinase subunits translocate with the DNA strands. Cell. 2004 Oct 1;119(1):33-45. PMID:15454079 doi:https://dx.doi.org/10.1016/j.cell.2004.09.010

References

Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Jaime Prilusky, Joel L. Sussman, Michal Harel

[1] West SC. The search for a human Holliday junction resolvase. Biochem Soc Trans. 2009 Jun;37(Pt 3):519-26. doi: 10.1042/BST0370519. PMID:19442245 doi:https://dx.doi.org/10.1042/BST0370519

[2] Haldenby S, White MF, Allers T. RecA family proteins in archaea: RadA and its cousins. Biochem Soc Trans. 2009 Feb;37(Pt 1):102-7. doi: 10.1042/BST0370102. PMID:19143611 doi:https://dx.doi.org/10.1042/BST0370102

[3] Wardell K, Haldenby S, Jones N, Liddell S, Ngo GHP, Allers T. RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator. DNA Repair (Amst). 2017 Jul;55:7-16. PMID:28501701 doi:10.1016/j.dnarep.2017.04.005

[4] Dhar G, Sanders ER, Johnson RC. Architecture of the hin synaptic complex during recombination: the recombinase subunits translocate with the DNA strands. Cell. 2004 Oct 1;119(1):33-45. PMID:15454079 doi:https://dx.doi.org/10.1016/j.cell.2004.09.010

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Resolvase

Function

3D structures of resolvase

References

Proteopedia Page Contributors and Editors (what is this?)

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