3bgw | pdb_00003bgw

The Structure Of A DnaB-Like Replicative Helicase And Its Interactions With Primase

spinqualitylabelsall models 3bgw, resolution 3.91Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3bgw is a 6 chain structure with sequence from Bacillus phage SPP1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.91Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q38152_BPSPP Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Helicases are essential enzymes for DNA replication, a fundamental process in all living organisms. The DnaB family are hexameric replicative helicases that unwind duplex DNA and coordinate with RNA primase and other proteins at the replication fork in prokaryotes. Here, we report the full-length crystal structure of G40P, a DnaB family helicase. The hexamer complex reveals an unusual architectural feature and a new type of assembly mechanism. The hexamer has two tiers: a three-fold symmetric N-terminal tier and a six-fold symmetric C-terminal tier. Monomers with two different conformations, termed cis and trans, come together to provide a topological solution for the dual symmetry within a hexamer. Structure-guided mutational studies indicate an important role for the N-terminal tier in binding primase and regulating primase-mediated stimulation of helicase activity. This study provides insights into the structural and functional interplay between G40P helicase and DnaG primase. The structure of a DnaB-family replicative helicase and its interactions with primase.,Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS Nat Struct Mol Biol. 2008 Jan;15(1):94-100. Epub 2007 Dec 23. PMID:18157148 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Helicase 3D structures

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