8am5 | pdb_00008am5

RCII/PSI complex, class 3

spinqualitylabelsall models 8am5, resolution 3.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8am5 is a 10 chain structure with sequence from Synechocystis sp. PCC 6803. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.1Å Ligands: 45D, BCR, CLA, ECH, EQ3, FE2, HEM, LHG, LMG, LMT, PHO, PQN, ZEX Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PSBA2_SYNY3 Publication Abstract from PubMed Robust oxygenic photosynthesis requires a suite of accessory factors to ensure efficient assembly and repair of the oxygen-evolving photosystem two (PSII) complex. The highly conserved Ycf48 assembly factor binds to the newly synthesized D1 reaction center polypeptide and promotes the initial steps of PSII assembly, but its binding site is unclear. Here we use cryo-electron microscopy to determine the structure of a cyanobacterial PSII D1/D2 reaction center assembly complex with Ycf48 attached. Ycf48, a 7-bladed beta propeller, binds to the amino-acid residues of D1 that ultimately ligate the water-oxidising Mn(4)CaO(5) cluster, thereby preventing the premature binding of Mn(2+) and Ca(2+) ions and protecting the site from damage. Interactions with D2 help explain how Ycf48 promotes assembly of the D1/D2 complex. Overall, our work provides valuable insights into the early stages of PSII assembly and the structural changes that create the binding site for the Mn(4)CaO(5) cluster. The Ycf48 accessory factor occupies the site of the oxygen-evolving manganese cluster during photosystem II biogenesis.,Zhao Z, Vercellino I, Knoppova J, Sobotka R, Murray JW, Nixon PJ, Sazanov LA, Komenda J Nat Commun. 2023 Aug 4;14(1):4681. doi: 10.1038/s41467-023-40388-6. PMID:37542031 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Photosystem II 3D structures

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