9gef | pdb_00009gef

Experimental localization of metal-binding sites reveals the role of metal ions in the delafloxacin-stabilized Streptococcus pneumoniae topoisomerase IV DNA cleavage complex

spinqualitylabelsall models 9gef, resolution 2.62Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9gef is a 6 chain structure with sequence from Escherichia coli and Streptococcus pneumoniae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.62Å Ligands: ACT, CL, K, MG, MPD, TE9 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Metal ions have important roles in supporting the catalytic activity of DNA-regulating enzymes such as topoisomerases (topos). Bacterial type II topos, gyrases and topo IV, are primary drug targets for fluoroquinolones, a class of clinically relevant antibacterials requiring metal ions for efficient drug binding. While the presence of metal ions in topos has been elucidated in biochemical studies, accurate location and assignment of metal ions in structural studies have historically posed significant challenges. Recent advances in X-ray crystallography address these limitations by extending the experimental capabilities into the long-wavelength range, exploiting the anomalous contrast from light elements of biological relevance. This breakthrough enables us to confirm experimentally the locations of Mg(2+) in the fluoroquinolone-stabilized Streptococcus pneumoniae topo IV complex. Moreover, we can unambiguously identify the presence of K(+) and Cl(-) ions in the complex with one pair of K(+) ions functioning as an additional intersubunit bridge. Overall, our data extend current knowledge on the functional and structural roles of metal ions in type II topos. Experimental localization of metal-binding sites reveals the role of metal ions in type II DNA topoisomerases.,Wang B, Najmudin S, Pan XS, Mykhaylyk V, Orr C, Wagner A, Govada L, Chayen NE, Fisher LM, Sanderson MR Proc Natl Acad Sci U S A. 2024 Oct 8;121(41):e2413357121. doi: , 10.1073/pnas.2413357121. Epub 2024 Oct 3. PMID:39361644 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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