7k7x | pdb_00007k7x
Solution structure of the cyclotide pase A
Structural highlights
Publication Abstract from PubMedCyclotides are plant-derived peptides found within five families of flowering plants (Violaceae, Rubiaceae, Fabaceae, Solanaceae, and Poaceae) that have a cyclic backbone and six conserved cysteine residues linked by disulfide bonds. Their presence within the Violaceae species seems ubiquitous, yet not all members of other families produce these macrocyclic peptides. The genus Palicourea Aubl. (Rubiaceae) contains hundreds of neotropical species of shrubs and small trees; however, only a few cyclotides have been discovered hitherto. Herein, five previously uncharacterized Mobius cyclotides within Palicourea sessilis and their pharmacological activities are described. Cyclotides were isolated from leaves and stems of this plant and identified as pase A-E, as well as the known peptide kalata S. Cyclotides were de novo sequenced by MALDI-TOF/TOF mass spectrometry, and their structures were solved by NMR spectroscopy. Because some cyclotides have been reported to modulate immune cells, pase A-D were assayed for cell proliferation of human primary activated T lymphocytes, and the results showed a dose-dependent antiproliferative function. The toxicity on other nonimmune cells was also assessed. This study reveals that pase cyclotides have potential for applications as immunosuppressants and in immune-related disorders. Cyclotides from Brazilian Palicourea sessilis and Their Effects on Human Lymphocytes.,Pinto MEF, Chan LY, Koehbach J, Devi S, Grundemann C, Gruber CW, Gomes M, Bolzani VS, Cilli EM, Craik DJ J Nat Prod. 2021 Jan 22;84(1):81-90. doi: 10.1021/acs.jnatprod.0c01069. Epub 2021, Jan 4. PMID:33397096[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
| ||||||||||||||||||
