7mxo | pdb_00007mxo

CryoEM structure of human NKCC1

spinqualitylabelsall models 7mxo, resolution 3.47Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7mxo is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.47Å Ligands: CL, K Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function S12A2_HUMAN Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Publication Abstract from PubMed The Na-K-2Cl cotransporter-1 (NKCC1) is an electroneutral Na(+)-dependent transporter responsible for simultaneously translocating Na(+), K(+), and Cl(-) ions into cells. In human tissue, NKCC1 plays a critical role in regulating cytoplasmic volume, fluid intake, chloride homeostasis, and cell polarity. Here, we report four structures of human NKCC1 (hNKCC1), both in the absence and presence of loop diuretic (bumetanide or furosemide), using single-particle cryo-electron microscopy. These structures allow us to directly observe various novel conformations of the hNKCC1 dimer. They also reveal two drug-binding sites located at the transmembrane and cytosolic carboxyl-terminal domains, respectively. Together, our findings enable us to delineate an inhibition mechanism that involves a coupled movement between the cytosolic and transmembrane domains of hNKCC1. Inhibition mechanism of NKCC1 involves the carboxyl terminus and long-range conformational coupling.,Moseng MA, Su CC, Rios K, Cui M, Lyu M, Glaza P, Klenotic PA, Delpire E, Yu EW Sci Adv. 2022 Oct 28;8(43):eabq0952. doi: 10.1126/sciadv.abq0952. Epub 2022 Oct , 28. PMID:36306358 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Solute carrier family 12 3D structures Symporter 3D structures

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