8rnx | pdb_00008rnx

Hen Egg White Lysozyme soaked with [HIsq][trans-RuCl4(DMSO)(Isq)]

spinqualitylabelsall models 8rnx, resolution 1.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8rnx is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.25Å Ligands: CL, EDO, NA, RU Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed This study presents a comprehensive structural analysis of the adducts formed upon the reaction of two Ru(III) complexes [HIsq][trans-Ru(III)Cl(4)(dmso)(Isq)] (1) and [H(2)Ind][trans-Ru(III)Cl(4)(dmso)(HInd)] (2) (where HInd-indazole, Isq-isoquinoline, analogs of NAMI-A) and two Ru(II) complexes, cis-[RuCl(2)(dmso)(4)] (c) and trans-[RuCl(2)(dmso)(4)] (t), with hen-egg white lysozyme (HEWL). Additionally, the crystal structure of an adduct of human lysozyme (HL) with ruthenium complex, [H(2)Ind][trans-RuCl(4)(dmso)(HInd)] was solved. X-ray crystallographic data analysis revealed that all studied Ru complexes, regardless of coordination surroundings and metal center charge, coordinate to the same amino acids (His15, Arg14, and Asp101) of HEWL, losing most of their original ligands. In the case of the 2-HL adduct, two distinct metalation sites: (i) Arg107, Arg113 and (ii) Gln127, Gln129, were identified. Crystallographic data were supported by studies of the interaction of 1 and 2 with HEWL in an aqueous solution. Hydrolytic stability studies revealed that both complexes 1 and 2 liberate the N-heterocyclic ligand under crystallization-like conditions (pH 4.5) as well as under physiological pH conditions, and this process is not significantly affected by the presence of HEWL. A comparative examination of nine crystal structures of Ru complexes with lysozyme, obtained through soaking and co-crystallization experiments, together with in-solution studies of the interaction between 1 and 2 with HEWL, indicates that the hydrolytic release of the N-heterocyclic ligand is one of the critical factors in the interaction between Ru complexes and lysozyme. This understanding is crucial in shedding light on the tendency of Ru complexes to target diverse metalation sites during the formation and in the final forms of the adducts with proteins. Exploring the coordination chemistry of ruthenium complexes with lysozymes: structural and in-solution studies.,Oszajca M, Flejszar M, Szura A, Drozdz P, Brindell M, Kurpiewska K Front Chem. 2024 Apr 4;12:1371637. doi: 10.3389/fchem.2024.1371637. eCollection , 2024. PMID:38638879 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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