8tt3 | pdb_00008tt3

S. thermodepolymerans KpsM-KpsE in Glycolipid 2 state with rigid body fitted KpsT

spinqualitylabelsall models 8tt3, resolution 3.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8tt3 is a 12 chain structure with sequence from Caldimonas thermodepolymerans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.4Å Ligands: KJ9 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Capsular polysaccharides (CPSs) fortify the cell boundaries of many commensal and pathogenic bacteria(1). Through the ABC-transporter-dependent biosynthesis pathway, CPSs are synthesized intracellularly on a lipid anchor and secreted across the cell envelope by the KpsMT ABC transporter associated with the KpsE and KpsD subunits(1,2). Here we use structural and functional studies to uncover crucial steps of CPS secretion in Gram-negative bacteria. We show that KpsMT has broad substrate specificity and is sufficient for the translocation of CPSs across the inner bacterial membrane, and we determine the cell surface organization and localization of CPSs using super-resolution fluorescence microscopy. Cryo-electron microscopy analyses of the KpsMT-KpsE complex in six different states reveal a KpsE-encaged ABC transporter, rigid-body conformational rearrangements of KpsMT during ATP hydrolysis and recognition of a glycolipid inside a membrane-exposed electropositive canyon. In vivo CPS secretion assays underscore the functional importance of canyon-lining basic residues. Combined, our analyses suggest a molecular model of CPS secretion by ABC transporters. Molecular insights into capsular polysaccharide secretion.,Kuklewicz J, Zimmer J Nature. 2024 Apr;628(8009):901-909. doi: 10.1038/s41586-024-07248-9. Epub 2024 , Apr 3. PMID:38570679 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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