8va7 | pdb_00008va7

Crystal structure of CapGH3a enzyme retrieved from capybara gut metagenome

spinqualitylabelsall models 8va7, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8va7 is a 2 chain structure with sequence from Gut metagenome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.6Å Ligands: GOL, PEG, PGE, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Gut microbiota members from the Bacteroidota phylum play a pivotal role in mammalian health and metabolism. They thrive in this diverse ecosystem due to their notable ability to cope with distinct recalcitrant dietary glycans via polysaccharide utilization loci (PULs). Our study reveals that a PUL from an herbivore gut bacterium belonging to the Bacteroidota phylum, with a gene composition similar to that in the human gut, exhibits extended functionality. While the human gut PUL targets mixed-linkage beta-glucans specifically, the herbivore gut PUL also efficiently processes linear and substituted beta-1,3-glucans. This gain of function emerges from molecular adaptations in recognition proteins and carbohydrate-active enzymes, including a beta-glucosidase specialized for beta(1,6)-glucosyl linkages, a typical substitution in beta(1,3)-glucans. These findings broaden the existing model for non-cellulosic beta-glucans utilization by gut bacteria, revealing an additional layer of functional and evolutionary complexity within the gut microbiota, beyond conventional gene insertions/deletions to intricate biochemical interactions. A functionally augmented carbohydrate utilization locus from herbivore gut microbiota fueled by dietary beta-glucans.,Mandelli F, Martins MP, Chinaglia M, Lima EA, Morais MAB, Lima TB, Cabral L, Pirolla RAS, Fuzita FJ, Paixao DAA, Andrade MO, Wolf LD, Vieira PS, Persinoti GF, Murakami MT NPJ Biofilms Microbiomes. 2024 Oct 14;10(1):105. doi: 10.1038/s41522-024-00578-6. PMID:39397008 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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