6fhw | pdb_00006fhw

Structure of Hormoconis resinae Glucoamylase

spinqualitylabelsall models 6fhw, resolution 3.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6fhw is a 2 chain structure with sequence from Amorphotheca resinae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.6Å Ligands: AC1, BMA, GLC, MAN, NAG, PRD_900007 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function AMYG_AMORE Publication Abstract from PubMed Glucoamylases are one of the most important classes of enzymes in the industrial degradation of starch biomass. They consist of a catalytic domain and a carbohydrate-binding domain (CBM), with the latter being important for the interaction with the polymeric substrate. Whereas the catalytic mechanisms and structures of the individual domains are well known, the spatial arrangement of the domains with respect to each other and its influence on activity are not fully understood. Here, the structures of three industrially used fungal glucoamylases, two of which are full length, have been crystallized and determined. It is shown for the first time that the relative orientation between the CBM and the catalytic domain is flexible, as they can adopt different orientations independently of ligand binding, suggesting a role as an anchor to increase the contact time and the relative concentration of substrate near the active site. The flexibility in the orientations of the two domains presented a considerable challenge for the crystallization of the enzymes. Structural insight into industrially relevant glucoamylases: flexible positions of starch-binding domains.,Roth C, Moroz OV, Ariza A, Skov LK, Ayabe K, Davies GJ, Wilson KS Acta Crystallogr D Struct Biol. 2018 May 1;74(Pt 5):463-470. doi:, 10.1107/S2059798318004989. Epub 2018 Apr 26. PMID:29717717 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Alpha-glucosidase 3D structures

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