6jil | pdb_00006jil
Crystal structure of D-cycloserine synthetase DcsG
Structural highlights
FunctionDCSG_STRLA Involved in the biosynthesis of the antibiotic D-cycloserine (DCS), a cyclic structural analog of D-alanine, used as an antitubercular agent. Catalyzes the synthesis of D-cycloserine from O-ureido-D-serine. It reacts exclusively with O-ureido-D-serine.[1] [2] Publication Abstract from PubMedIn the biosynthetic pathway of an anti-tubercular antibiotic D-cycloserine (D-CS), O-ureido-D-serine (D-OUS) is converted to D-CS. We have previously demonstrated that DcsG, classified into the ATP-grasp superfamily enzyme, catalyzes the ring formation to generate D-CS, which is accompanied by the cleavage of a bond in the urea moiety of D-OUS to remove a carbamoyl group. Although the general ATP-grasp enzymes catalyze an ATP-dependent ligation reaction between two substrates, DcsG catalyzes specifically the generation of an intramolecular covalent bond. In the present study, cyanate was found in the reaction mixture, suggesting that carbamoyl group is eliminated as an isocyanic acid during the reaction. By the crystallographic and mutational investigations of DcsG, we anticipate the residues necessary for the binding of D-OUS. An acylphosphate intermediate must be bound at the narrow pocket of DcsG in a folded conformation, inducing the bond cleavage and the new bond formation to generate cyanate and D-CS, respectively. Cyclization mechanism catalyzed by an ATP-grasp enzyme essential for D-cycloserine biosynthesis.,Matoba Y, Uda N, Kudo M, Sugiyama M FEBS J. 2019 Dec 3. doi: 10.1111/febs.15163. PMID:31793174[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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