7zcb | pdb_00007zcb

Human Pikachurin/EGFLAM N-terminal Fibronectin-III (1-2) domains

spinqualitylabelsall models 7zcb, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7zcb is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.5Å Ligands: BMA, CL, NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function EGFLA_HUMAN Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Plays a key role in the synaptic organization of photoreceptors by mediating transsynaptic interaction between alpha-dystroglycan and GPR179 on the postsynaptic membrane. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.[UniProtKB:Q4VBE4] Publication Abstract from PubMed Precise synapse formation is essential for normal functioning of the nervous system. Retinal photoreceptors establish selective contacts with bipolar cells, aligning the neurotransmitter release apparatus with postsynaptic signaling cascades. This involves transsynaptic assembly between the dystroglycan-dystrophin complex on the photoreceptor and the orphan receptor GPR179 on the bipolar cell, which is mediated by the extracellular matrix protein pikachurin (also known as EGFLAM). This complex plays a critical role in the synaptic organization of photoreceptors and signal transmission, and mutations affecting its components cause blinding disorders in humans. Here, we investigated the structural organization and molecular mechanisms by which pikachurin orchestrates transsynaptic assembly and solved structures of the human pikachurin domains by x-ray crystallography and of the GPR179-pikachurin complex by single-particle, cryo-electron microscopy. The structures reveal molecular recognition principles of pikachurin by the Cache domains of GPR179 and show how the interaction is involved in the transsynaptic alignment of the signaling machinery. Together, these data provide a structural basis for understanding the synaptic organization of photoreceptors and ocular pathology. Structure of the photoreceptor synaptic assembly of the extracellular matrix protein pikachurin with the orphan receptor GPR179.,Patil DN, Pantalone S, Cao Y, Laboute T, Novick SJ, Singh S, Savino S, Faravelli S, Magnani F, Griffin PR, Singh AK, Forneris F, Martemyanov KA Sci Signal. 2023 Jul 25;16(795):eadd9539. doi: 10.1126/scisignal.add9539. Epub , 2023 Jul 25. PMID:37490546 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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