3qac | pdb_00003qac

Structure of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.

spinqualitylabelsall models 3qac, resolution 2.27Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3qac is a 1 chain structure with sequence from Amaranthus hypochondriacus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.275Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q38712_AMAHP Publication Abstract from PubMed 11S globulin is one of the major seed storage proteins in amaranth. Recombinant protein was produced as up to approximately 80% of the total bacterial protein using Escherichia coli Rosetta-gami (DE3) containing pET21d with amaranth 11S globulin cDNA. The best expression condition was at 302 K for 20 h using LB medium containing 0.5 M NaCl. The recombinant protein was easily separated from most of the Escherichia coli proteins by precipitation with 0-40% ammonium sulfate solution. It formed aggregates at low temperature and at low salt concentrations. This behaviour may imply that it has a more hydrophobic nature than other 11S seed globulins. The crystals diffracted to 6 A resolution and belonged to space group P6(3), with unit-cell parameters a=b=97.6, c=74.8 A, gamma=120.0 degrees. One subunit of a trimer was estimated to be present in the asymmetric unit, assuming a Vsol of 41%. To obtain the complete structure solution, experiments to improve crystallization and flash-cooling conditions are in progress. Expression, purification and preliminary crystallization of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.,Tandang-Silvas MR, Carrazco-Pena L, Barba de la Rosa AP, Osuna-Castro JA, Utsumi S, Mikami B, Maruyama N Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt, 8):919-22. Epub 2010 Jul 29. PMID:020693668 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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