5b5i | pdb_00005b5i

The crystal structure of a crustacean hyperglycemic hormone precursor from the kuruma prawn

spinqualitylabelsall models 5b5i, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5b5i is a 2 chain structure with sequence from Penaeus japonicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.599Å Ligands: EOH Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CHH1_PENJP Hormone found in the sinus gland of isopods and decapods which controls the blood sugar level. Has a secretagogue action over the amylase released from the midgut gland. May act as a stress hormone and may be involved in the control of molting and reproduction. Publication Abstract from PubMed The crustacean hyperglycemic hormone (CHH) is one of the major hormones in crustaceans, and peptides belonging to the CHH superfamily have been found in diverse ecdysozoans. Although the basic function of CHH is to control energy metabolism, it also plays various roles in crustacean species, such as in molting and vitellogenesis. Here, we present the crystal structure of Pej-SGP-I-Gly, a partially active precursor of CHH from the kuruma prawn Marsupenaeus japonicus, which has an additional Gly residue in place of the C-terminal amide group of the mature Pej-SGP-I. The 1.6-angstrom crystal structure showed not only the common CHH superfamily scaffold comprising three alpha-helices, three disulfide bridges, and a hydrophobic core, but also revealed that the C-terminal part has a variant backbone fold that is specific to Pej-SGP-I-Gly. The alpha-helix 4 of Pej-SGP-I-Gly was much longer than that of molt-inhibiting hormone (Pej-MIH) from the same species, and as a result, the following C-terminal helix, corresponding to alpha-helix 5 in MIH, was not formed. Unlike monomeric Pej-MIH, Pej-SGP-I-Gly forms a homodimer in the crystal structure via its unique alpha-helix 4. The unexpected dissimilar folds between Pej-SGP-I-Gly and Pej-MIH appear to be the result of their distinct C-terminal amino acid sequences. Variations in amino acid sequences and lengths and the resulting variety of backbone folds allow the C-terminal and sterically adjoining regions to confer different hormonal activities in diverse CHH superfamily members. This article is protected by copyright. All rights reserved. Crystal structure of a crustacean hyperglycemic hormone (CHH) precursor suggests structural variety in the C-terminal regions of CHH superfamily members.,Tsutsui N, Sakamoto T, Arisaka F, Tanokura M, Nagasawa H, Nagata K FEBS J. 2016 Oct 15. doi: 10.1111/febs.13926. PMID:27743429 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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