6xkt | pdb_00006xkt

R. capsulatus cyt bc1 with both FeS proteins in c position (CIII2 c-c)

spinqualitylabelsall models 6xkt, resolution 3.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6xkt is a 6 chain structure with sequence from Rhodobacter capsulatus SB 1003. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.75Å Ligands: FES, HEC Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CYB_RHOCB Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. Publication Abstract from PubMed Respiratory electron transport complexes are organized as individual entities or combined as large supercomplexes (SC). Gram-negative bacteria deploy a mitochondrial-like cytochrome (cyt) bc(1) (Complex III, CIII(2)), and may have specific cbb(3)-type cyt c oxidases (Complex IV, CIV) instead of the canonical aa(3)-type CIV. Electron transfer between these complexes is mediated by soluble (c(2)) and membrane-anchored (c(y)) cyts. Here, we report the structure of an engineered bc(1)-cbb(3) type SC (CIII(2)CIV, 5.2 A resolution) and three conformers of native CIII(2) (3.3 A resolution). The SC is active in vivo and in vitro, contains all catalytic subunits and cofactors, and two extra transmembrane helices attributed to cyt c(y) and the assembly factor CcoH. The cyt c(y) is integral to SC, its cyt domain is mobile and it conveys electrons to CIV differently than cyt c(2). The successful production of a native-like functional SC and determination of its structure illustrate the characteristics of membrane-confined and membrane-external respiratory electron transport pathways in Gram-negative bacteria. Cryo-EM structures of engineered active bc(1)-cbb(3) type CIII(2)CIV super-complexes and electronic communication between the complexes.,Steimle S, van Eeuwen T, Ozturk Y, Kim HJ, Braitbard M, Selamoglu N, Garcia BA, Schneidman-Duhovny D, Murakami K, Daldal F Nat Commun. 2021 Feb 10;12(1):929. doi: 10.1038/s41467-021-21051-4. PMID:33568648 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Cytochrome C 3D structures

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