7yfz | pdb_00007yfz

Cyanophage Pam3 baseplate proteins

spinqualitylabelsall models 7yfz, resolution 3.19Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7yfz is a 42 chain structure with sequence from Uncultured cyanophage. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.19Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A9E7DTG9_9CAUD Publication Abstract from PubMed The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail contraction mechanism of myophage remain largely unknown. Here, we present the fine structure of a freshwater Myoviridae cyanophage Pam3, which has an icosahedral capsid of ~680 A in diameter, connected via a three-section neck to an 840-A-long contractile tail, ending with a three-module baseplate composed of only six protein components. This simplified baseplate consists of a central hub-spike surrounded by six wedge heterotriplexes, to which twelve tail fibers are covalently attached via disulfide bonds in alternating upward and downward configurations. In vitro reduction assays revealed a putative redox-dependent mechanism of baseplate assembly and tail sheath contraction. These findings establish a minimal myophage that might become a user-friendly chassis phage in synthetic biology. Fine structure and assembly pattern of a minimal myophage Pam3.,Yang F, Jiang YL, Zhang JT, Zhu J, Du K, Yu RC, Wei ZL, Kong WW, Cui N, Li WF, Chen Y, Li Q, Zhou CZ Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2213727120. doi: , 10.1073/pnas.2213727120. Epub 2023 Jan 19. PMID:36656854 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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