9auq | pdb_00009auq

Crystal structure of 4-Fluoro-tryptophan labeled Oscillatoria Agardhii agglutinin

spinqualitylabelsall models 9auq, resolution 2.37Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9auq is a 2 chain structure with sequence from Planktothrix agardhii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.37Å Ligands: 4FW, CXS Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function C0STD7_PLAAG Publication Abstract from PubMed Traditional protein structure determination by magic angle spinning (MAS) solid-state NMR spectroscopy primarily relies on interatomic distances up to 8 A, extracted from (13)C-, (15)N-, and (1)H-based dipolar-based correlation experiments. Here, we show that (19)F fast (60 kHz) MAS NMR spectroscopy can supply additional, longer distances. Using 4F-Trp,U-(13)C,(15)N crystalline Oscillatoria agardhii agglutinin (OAA), we demonstrate that judiciously designed 2D and 3D (19)F-based dipolar correlation experiments such as (H)CF, (H)CHF, and FF can yield interatomic distances in the 8-16 A range. Incorporation of fluorine-based restraints into structure calculation improved the precision of Trp side chain conformations as well as regions in the protein around the fluorine containing residues, with notable improvements observed for residues in proximity to the Trp pairs (W10/W17 and W77/W84) in the carbohydrate-binding loops, which lacked sufficient long-range (13)C-(13)C distance restraints. Our work highlights the use of fluorine and (19)F fast MAS NMR spectroscopy as a powerful structural biology tool. Integrating (19)F Distance Restraints for Accurate Protein Structure Determination by Magic Angle Spinning NMR Spectroscopy.,Runge BR, Zadorozhnyi R, Quinn CM, Russell RW, Lu M, Antolinez S, Struppe J, Schwieters CD, Byeon IL, Hadden-Perilla JA, Gronenborn AM, Polenova T J Am Chem Soc. 2024 Oct 23. doi: 10.1021/jacs.4c11373. PMID:39440810 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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