4a7p | pdb_00004a7p

Se-Met derivatized UgdG, UDP-glucose dehydrogenase from Sphingomonas elodea

spinqualitylabelsall models 4a7p, resolution 3.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4a7p is a 2 chain structure with sequence from Sphingomonas elodea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.403Å Ligands: MSE, NAD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A4UTT2_SPHEL Publication Abstract from PubMed Gellan gum, a commercial gelling agent produced by Sphingomonas elodea ATCC 31461, is a high-value microbial exopolysaccharide. UDP-glucose dehydrogenase (UGD; EC 1.1.1.22) is responsible for the NAD-dependent twofold oxidation of UDP-glucose to UDP-glucuronic acid, one of the key components for gellan biosynthesis. S. elodea ATCC 31461 UGD, termed UgdG, was cloned, expressed, purified and crystallized in native and SeMet-derivatized forms in hexagonal and tetragonal space groups, respectively; the crystals diffracted X-rays to 2.40 and 3.40 A resolution, respectively. Experimental phases were obtained for the tetragonal SeMet-derivatized crystal form by a single-wavelength anomalous dispersion experiment. This structure was successfully used as a molecular-replacement probe for the hexagonal crystal form of the native protein. Cloning, expression, purification, crystallization and preliminary crystallographic studies of UgdG, an UDP-glucose dehydrogenase from Sphingomonas elodea ATCC 31461.,Rocha J, Granja AT, Sa-Correia I, Fialho A, Frazao C Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt, 1):69-72. Epub 2009 Dec 25. PMID:020057075 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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