7qop | pdb_00007qop

A mutant of the nitrile hydratase from Geobacillus pallidus having enhanced thermostability

spinqualitylabelsall models 7qop, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7qop is a 2 chain structure with sequence from Aeribacillus pallidus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: CL, CO, CSD, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q84FS5_9BACI Publication Abstract from PubMed Nitrile hydratases (NHases) are important biocatalysts for the enzymatic conversion of nitriles to industrially-important amides such as acrylamide and nicotinamide. Although thermostability in this enzyme class is generally low, there is not sufficient understanding of its basis for rational enzyme design. The gene expressing the Co-type NHase from the moderate thermophile, Geobacillus pallidus RAPc8 (NRRL B-59396), was subjected to random mutagenesis. Four mutants were selected that were 3 to 15-fold more thermostable than the wild-type NHase, resulting in a 3.4-7.6 ​kJ/mol increase in the activation energy of thermal inactivation at 63 ​ degrees C. High resolution X-ray crystal structures (1.15-1.80 ​A) were obtained of the wild-type and four mutant enzymes. Mutant 9E, with a resolution of 1.15 ​A, is the highest resolution crystal structure obtained for a nitrile hydratase to date. Structural comparisons between the wild-type and mutant enzymes illustrated the importance of salt bridges and hydrogen bonds in enhancing NHase thermostability. These additional interactions variously improved thermostability by increased intra- and inter-subunit interactions, preventing cooperative unfolding of alpha-helices and stabilising loop regions. Some hydrogen bonds were mediated via a water molecule, specifically highlighting the significance of structured water molecules in protein thermostability. Although knowledge of the mutant structures makes it possible to rationalize their behaviour, it would have been challenging to predict in advance that these mutants would be stabilising. Engineering enhanced thermostability into the Geobacillus pallidus nitrile hydratase.,Van Wyk JC, Sewell BT, Danson MJ, Tsekoa TL, Sayed MF, Cowan DA Curr Res Struct Biol. 2022 Aug 19;4:256-270. doi: 10.1016/j.crstbi.2022.07.002. , eCollection 2022. PMID:36106339 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Nitrile hydratase

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