3vm7 | pdb_00003vm7

Structure of an Alpha-Amylase from Malbranchea cinnamomea

spinqualitylabelsall models 3vm7, resolution 2.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3vm7 is a 1 chain structure with sequence from Malbranchea cinnamomea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.25Å Ligands: CA, GLC, NAG, TRS Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function K9L8F3_MALCI Publication Abstract from PubMed A novel alpha-amylase (McAmyA) from the thermophilic fungus, Malbranchea cinnamomea was purified, characterized and crystallized in the present study. McAmyA was purified to apparent homogeneity with a molecular mass of 60.3 kDa on SDS-PAGE. The enzyme exhibited maximal activity at pH 6.5 and was stable within pH 5.0-10.0. It was most active at 65 degrees C and was stable up to 50 degrees C. McAmyA was capable of hydrolyzing amylose, starch, amylopectin, pullulan, cyclodextrins and maltooligosaccharides. The full-length cDNA of an alpha-amylase gene (McAmyA) from the strain was cloned. McAmyA consisted of a 1,476-bp open reading frame encoding 492 amino acids. It displayed the highest amino acid sequence homology (less than 60 %) with the reported alpha-amylases. The crystal structure of McAmyA was solved at a resolution of 2.25 A (PDB code 3VM7). The overall structure of McAmyA reveals three domains with ten alpha helices and 14 beta strands, and the putative catalytic residues are positioned at domain A with somewhat different secondary structural circumstances compared with typical alpha-amylases. A novel multifunctional alpha-amylase from the thermophilic fungus Malbranchea cinnamomea: biochemical characterization and three-dimensional structure.,Han P, Zhou P, Hu S, Yang S, Yan Q, Jiang Z Appl Biochem Biotechnol. 2013 May;170(2):420-35. doi: 10.1007/s12010-013-0198-y. , Epub 2013 Mar 29. PMID:23536251 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Amylase 3D structures

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