4uoi | pdb_00004uoi

Unexpected structure for the N-terminal domain of Hepatitis C virus envelope glycoprotein E1

spinqualitylabelsall models 4uoi, resolution 3.49Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4uoi is a 6 chain structure with sequence from Hepatitis C virus (isolate H77). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.49Å Ligands: NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function H9XGD6_9HEPC Publication Abstract from PubMed Single-wavelength anomalous dispersion of S atoms (S-SAD) is an elegant phasing method to determine crystal structures that does not require heavy-atom incorporation or selenomethionine derivatization. Nevertheless, this technique has been limited by the paucity of the signal at the usual X-ray wavelengths, requiring very accurate measurement of the anomalous differences. Here, the data collection and structure solution of the N-terminal domain of the ectodomain of HCV E1 from crystals that diffracted very weakly is reported. By combining the data from 32 crystals, it was possible to solve the sulfur substructure and calculate initial maps at 7 A resolution, and after density modication and phase extension using a higher resolution native data set to 3.5 A resolution model building was achievable. Pushing the limits of sulfur SAD phasing: de novo structure solution of the N-terminal domain of the ectodomain of HCV E1.,El Omari K, Iourin O, Kadlec J, Fearn R, Hall DR, Harlos K, Grimes JM, Stuart DI Acta Crystallogr D Biol Crystallogr. 2014 Aug 1;70(Pt 8):2197-203. doi:, 10.1107/S139900471401339X. Epub 2014 Jul 25. PMID:25084338 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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