6apl | pdb_00006apl

Crystal Structure of human ST6GALNAC2 in complex with CMP

spinqualitylabelsall models 6apl, resolution 2.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6apl is a 6 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.35Å Ligands: C5P, MSE, NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SIA7B_HUMAN Publication Abstract from PubMed Vertebrate glycoproteins and glycolipids are synthesized in complex biosynthetic pathways localized predominantly within membrane compartments of the secretory pathway. The enzymes that catalyze these reactions are exquisitely specific, yet few have been extensively characterized because of challenges associated with their recombinant expression as functional products. We used a modular approach to create an expression vector library encoding all known human glycosyltransferases, glycoside hydrolases, and sulfotransferases, as well as other glycan-modifying enzymes. We then expressed the enzymes as secreted catalytic domain fusion proteins in mammalian and insect cell hosts, purified and characterized a subset of the enzymes, and determined the structure of one enzyme, the sialyltransferase ST6GalNAcII. Many enzymes were produced at high yields and at similar levels in both hosts, but individual protein expression levels varied widely. This expression vector library will be a transformative resource for recombinant enzyme production, broadly enabling structure-function studies and expanding applications of these enzymes in glycochemistry and glycobiology. Expression system for structural and functional studies of human glycosylation enzymes.,Moremen KW, Ramiah A, Stuart M, Steel J, Meng L, Forouhar F, Moniz HA, Gahlay G, Gao Z, Chapla D, Wang S, Yang JY, Prabhakar PK, Johnson R, Rosa MD, Geisler C, Nairn AV, Seetharaman J, Wu SC, Tong L, Gilbert HJ, LaBaer J, Jarvis DL Nat Chem Biol. 2017 Dec 18. pii: nchembio.2539. doi: 10.1038/nchembio.2539. PMID:29251719 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Sialyltransferase 3D structures

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