8v2z | pdb_00008v2z

Cryo-EM Structure of Smooth Muscle Gamma Actin (ACTG2) Mutant R257C

spinqualitylabelsall models 8v2z, resolution 2.72Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8v2z is a 5 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.72Å Ligands: ADP, HIC, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Disease ACTH_HUMAN Myopathic intestinal pseudoobstruction;Megacystis-microcolon-intestinal hypoperistalsis syndrome;Familial visceral myopathy. The disease is caused by mutations affecting the gene represented in this entry. Function ACTH_HUMAN Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Publication Abstract from PubMed Visceral myopathy is a life-threatening disease characterized by muscle weakness in the bowel, bladder, and uterus. Mutations in smooth muscle gamma-actin (ACTG2) are the most common cause of the disease, but the mechanisms by which the mutations alter muscle function are unknown. Here, we examined four prevalent ACTG2 mutations (R40C, R148C, R178C, and R257C) that cause different disease severity and are spread throughout the actin fold. R178C displayed premature degradation, R148C disrupted interactions with actin-binding proteins, R40C inhibited polymerization, and R257C destabilized filaments. Because these mutations are heterozygous, we also analyzed 50/50 mixtures with wild-type (WT) ACTG2. The WT/R40C mixture impaired filament nucleation by leiomodin 1, and WT/R257C produced filaments that were easily fragmented by smooth muscle myosin. Smooth muscle tropomyosin isoform Tpm1.4 partially rescued the defects of R40C and R257C. Cryo-electron microscopy structures of filaments formed by R40C and R257C revealed disrupted intersubunit contacts. The biochemical and structural properties of the mutants correlate with their genotype-specific disease severity. Molecular mechanisms linking missense ACTG2 mutations to visceral myopathy.,Ceron RH, Baez-Cruz FA, Palmer NJ, Carman PJ, Boczkowska M, Heuckeroth RO, Ostap EM, Dominguez R Sci Adv. 2024 May 31;10(22):eadn6615. doi: 10.1126/sciadv.adn6615. Epub 2024 May , 31. PMID:38820162 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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