8jg7 | pdb_00008jg7
Serine decarboxylase
Structural highlights
FunctionSDC1_ARATH Catalyzes the biosynthesis of ethanolamine from serine. Highly specific for L-serine and does not attack D-serine, L-phosphoserine, phosphatidylserine, L-histidine L-glutamate L-tyrosine or L-tryptophan. Decarboxylation of free serine is the major source of ethanolamine production in plants and ethanolamine metabolism is crucial for the synthesis of choline, phosphatidylethanolamine (PE) and phosphatidylcholine (PC), and thus for plant growth.[1] [2] [3] Publication Abstract from PubMedEthylamine (EA), the precursor of theanine biosynthesis, is synthesized from alanine decarboxylation by alanine decarboxylase (AlaDC) in tea plants. AlaDC evolves from serine decarboxylase (SerDC) through neofunctionalization and has lower catalytic activity. However, lacking structure information hinders the understanding of the evolution of substrate specificity and catalytic activity. In this study, we solved the X-ray crystal structures of AlaDC from Camellia sinensis (CsAlaDC) and SerDC from Arabidopsis thaliana (AtSerDC). Tyr(341) of AtSerDC or the corresponding Tyr(336) of CsAlaDC is essential for their enzymatic activity. Tyr(111) of AtSerDC and the corresponding Phe(106) of CsAlaDC determine their substrate specificity. Both CsAlaDC and AtSerDC have a distinctive zinc finger and have not been identified in any other Group II PLP-dependent amino acid decarboxylases. Based on the structural comparisons, we conducted a mutation screen of CsAlaDC. The results indicated that the mutation of L110F or P114A in the CsAlaDC dimerization interface significantly improved the catalytic activity by 110% and 59%, respectively. Combining a double mutant of CsAlaDC(L110F/P114A) with theanine synthetase increased theanine production 672% in an in vitro system. This study provides the structural basis for the substrate selectivity and catalytic activity of CsAlaDC and AtSerDC and provides a route to more efficient biosynthesis of theanine. Structure and evolution of alanine/serine decarboxylases and the engineering of theanine production.,Wang H, Zhu B, Qiao S, Dong C, Wan X, Gong W, Zhang Z Elife. 2024 Sep 17;12:RP91046. doi: 10.7554/eLife.91046. PMID:39287621[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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