9i86 | pdb_00009i86

Enterobacteriaphage PRD1 - P12 protein filament in complex with poly(dT) ssDNA

spinqualitylabelsall models 9i86, resolution 2.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9i86 is a 8 chain structure with sequence from Enterobacteria phage PRD1 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.75Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function VP12_BPPRD Binds to single-stranded DNA (ssDNA). Has a regulatory effect on phage DNA metabolism and transcription of early genes. Publication Abstract from PubMed Protein-primed DNA replication is a unique mechanism, bioorthogonal to other known DNA replication modes. It relies on specialised single-stranded DNA (ssDNA)-binding proteins (SSBs) to stabilise ssDNA intermediates by unknown mechanisms. Here, we present the structural and biochemical characterisation of P12, an SSB from bacteriophage PRD1. High-resolution cryo-electron microscopy reveals that P12 forms a unique, cooperative filament along ssDNA. Each protomer binds the phosphate backbone of 6 nucleotides in a sequence-independent manner, protecting ssDNA from nuclease degradation. Filament formation is driven by an intrinsically disordered C-terminal tail, facilitating cooperative binding. We identify residues essential for ssDNA interaction and link the ssDNA-binding ability of P12 to toxicity in host cells. Bioinformatic analyses place the P12 fold as a distinct branch within the OB-like fold family. This work offers new insights into protein-primed DNA replication and lays a foundation for biotechnological applications. Structural basis for cooperative ssDNA binding by bacteriophage protein filament P12.,Trager LK, Degen M, Pereira J, Durairaj J, Teixeira RD, Hiller S, Huguenin-Dezot N Nucleic Acids Res. 2025 Feb 27;53(5):gkaf132. doi: 10.1093/nar/gkaf132. PMID:40052821 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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