9eis | pdb_00009eis

Ethylene forming enzyme in complex with manganese and 2-oxoglutarate from Penicillium digitatum

spinqualitylabelsall models 9eis, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9eis is a 4 chain structure with sequence from Penicillium digitatum Pd1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.8Å Ligands: AKG, CL, EDO, MN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A7T7BQH3_PENDI Publication Abstract from PubMed The ethylene-forming enzyme (EFE) from the fungus Penicillium digitatum strain Pd1 was heterologously produced in Escherichia coli and its properties were compared to the extensively characterized bacterial enzyme from Pseudomonas savastanoi strain PK2. Both enzymes catalyze four reactions: the conversion of 2-oxoglutarate (2OG) to ethylene and CO(2), oxidative decarboxylation of 2OG coupled to l-arginine (l-Arg) hydroxylation, uncoupled oxidative decarboxylation of 2OG, and the production of 3-hydroxypropionate (3-HP) from 2OG. The strain Pd1 enzyme exhibited a greater ratio of ethylene production over l-Arg hydroxylation than the PK2 strain EFE. The uncoupled decarboxylation of 2OG and 3-HP production are minor reactions in both cases, but they occur to a greater extent using the fungal enzyme. Additional distinctions of the fungal versus bacterial enzyme are noted in the absorbance maxima and l-Arg dependence of their anaerobic electronic spectra. The structures of the Pd1 EFE apoprotein and the EFE.Mn(II).2OG complex resembled the corresponding structures of the PK2 enzyme, but notable structural differences were observed in the computationally predicted Pd1 EFE.Fe(II).2OG.l-Arg complex versus the PK2 EFE.Mn(II).2OG.l-Arg crystal structure. These studies extend our biochemical understanding and represent the first structural and conformational characterization of a eukaryotic EFE. Biochemical, Structural, and Conformational Characterization of a Fungal Ethylene-Forming Enzyme.,Chatterjee S, Rankin JA, Farrugia MA, J S Rifayee SB, Christov CZ, Hu J, Hausinger RP Biochemistry. 2025 Mar 7. doi: 10.1021/acs.biochem.5c00038. PMID:40052306 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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