5uts | pdb_00005uts

Carbon Sulfoxide lyase, Egt2 in the Ergothioneine biosynthesis pathway

spinqualitylabelsall models 5uts, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5uts is a 8 chain structure with sequence from Neurospora crassa OR74A. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.303Å Ligands: FMT, LLP, MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function EGT2_NEUCR Publication Abstract from PubMed Sulfur incorporation in the biosynthesis of ergothioneine, a histidine thiol derivative, differs from other well-characterized transsulfurations. A combination of a mononuclear non-heme iron enzyme-catalyzed oxidative C-S bond formation and a subsequent pyridoxal 5'-phosphate (PLP)-mediated C-S lyase reaction leads to the net transfer of a sulfur atom from a cysteine to a histidine. In this study, we structurally and mechanistically characterized a PLP-dependent C-S lyase Egt2, which mediates the sulfoxide C-S bond cleavage in ergothioneine biosynthesis. A cation-pi interaction between substrate and enzyme accounts for Egt2's preference of sulfoxide over thioether as a substrate. Using mutagenesis and structural biology, we captured three distinct states of the Egt2 C-S lyase reaction cycle, including a labile sulfenic intermediate captured in Egt2 crystals. Chemical trapping and high-resolution mass spectrometry were used to confirm the involvement of the sulfenic acid intermediate in Egt2 catalysis. Snapshots of C-S Cleavage in Egt2 Reveals Substrate Specificity and Reaction Mechanism.,Irani S, Naowarojna N, Tang Y, Kathuria KR, Wang S, Dhembi A, Lee N, Yan W, Lyu H, Costello CE, Liu P, Zhang YJ Cell Chem Biol. 2018 May 17;25(5):519-529.e4. doi:, 10.1016/j.chembiol.2018.02.002. Epub 2018 Mar 1. PMID:29503207 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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