6oic | pdb_00006oic

Crystal structure of human Sulfide Quinone Oxidoreductase in complex with coenzyme Q (sulfite soaked)

spinqualitylabelsall models 6oic, resolution 2.21Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6oic is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.21Å Ligands: FAD, H2S, UQ1 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SQOR_HUMAN Catalyzes the oxidation of hydrogen sulfide with the help of a quinone, such as ubiquinone, giving rise to thiosulfate and ultimately to sulfane (molecular sulfur) atoms. Requires an additional electron acceptor; can use sulfite, sulfide or cyanide (in vitro). Publication Abstract from PubMed Mitochondrial sulfide quinone oxidoreductase (SQR) catalyzes the oxidation of H2S to glutathione persulfide with concomitant reduction of CoQ10. We report herein that the promiscuous activity of human SQR supported the conversion of CoA to CoA-SSH (CoA-persulfide), a potent inhibitor of butyryl-CoA dehydrogenase, and revealed a molecular link between sulfide and butyrate metabolism, which are known to interact. Three different CoQ1-bound crystal structures furnished insights into how diverse substrates access human SQR, and provided snapshots of the reaction coordinate. Unexpectedly, the active site cysteines in SQR are configured in a bridging trisulfide at the start and end of the catalytic cycle, and the presence of sulfane sulfur was confirmed biochemically. Importantly, our study leads to a mechanistic proposal for human SQR in which sulfide addition to the trisulfide cofactor eliminates (201)Cys-SSH, forming an intense charge-transfer complex with flavin adenine dinucleotide, and (379)Cys-SSH, which transfers sulfur to an external acceptor. A Catalytic Trisulfide in Human Sulfide Quinone Oxidoreductase Catalyzes Coenzyme A Persulfide Synthesis and Inhibits Butyrate Oxidation.,Landry AP, Moon S, Kim H, Yadav PK, Guha A, Cho US, Banerjee R Cell Chem Biol. 2019 Nov 21;26(11):1515-1525.e4. doi:, 10.1016/j.chembiol.2019.09.010. Epub 2019 Oct 4. PMID:31591036 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Quinone reductase 3D structures 3D structures of sulfide quinone oxidoreductase

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