9kar | pdb_00009kar

Solution NMR structure of a new lasso peptide streptacidin

spinqualitylabelsall models 9kar Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9kar is a 1 chain structure with sequence from Streptacidiphilus jiangxiensis. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR, 15 models Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed A lasso peptide biosynthetic gene cluster (BGC) was identified through genome mining in the species Streptacidiphilus jiangxiensis CGMCC 4.1857, which was isolated from acidic rhizosphere soil. The BGC was reconstructed in Escherichia coli, leading to the heterologous production of a lasso peptide named streptacidin. The solution structure of streptacidin was determined based on the distance constraints derived from the NOESY spectrum, establishing the first lasso peptide utilizing methionine as a steric lock for the unique spatial configuration and the remarkable stability against heat (up to 95 degrees C) and proteolytic degradation by carboxypeptidase Y and chymotrypsin. Under experimental conditions, streptacidin showed weak effectiveness against vancomycin sensitive Enterococcus faecalis ATCC 29212 and Pseudomonas aeruginosa ATCC 902. These findings further the understanding of this distinctive class of compounds. Genome mining and heterologous expression reveal streptacidin, a new lasso peptide from Streptacidiphilus jiangxiensis.,Huang J, Cheng B, Cao C, Liu W Org Biomol Chem. 2025 Feb 12;23(7):1723-1730. doi: 10.1039/d4ob01998a. PMID:39807060 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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