9kjb | pdb_00009kjb

Crystal structure of Anti-CRISPR AcrIE7

spinqualitylabelsall models 9kjb, resolution 2.11Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9kjb is a 1 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.11Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The CRISPR-Cas system is a well-known adaptive immune system in bacteria, and a prominent mechanism for evading this immunity involves anti-CRISPR (Acr) proteins, which employ various methods to neutralize the CRISPR-Cas system. In this study, using structural and biochemical analyses, we revealed that AcrIE7 binds to the single-stranded DNA in the R-loop formed when Cascade encounters the target DNA, thereby preventing Cas3 from cleaving the DNA. This represents a different inhibition strategy distinct from previously reported Acr mechanisms and offers insights into CRISPR-Cas inhibition. AcrIE7 inhibits the CRISPR-Cas system by directly binding to the R-loop single-stranded DNA.,Kim DY, Lee SY, Ha HJ, Park HH Proc Natl Acad Sci U S A. 2025 Apr 8;122(14):e2423205122. doi: , 10.1073/pnas.2423205122. Epub 2025 Apr 3. PMID:40178896 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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