6m0p | pdb_00006m0p

Hydroxylamine oxidoreductase in complex with juglone

spinqualitylabelsall models 6m0p, resolution 2.78Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6m0p is a 6 chain structure with sequence from Nitrosomonas europaea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.78Å Ligands: 1PE, HEC, ISW, JUG, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function HAO_NITEU Publication Abstract from PubMed Nitrification, the microbial conversion of ammonia to nitrate via nitrite, plays a pivotal role in the global nitrogen cycle. However, the excessive use of ammonium-based fertilizers in agriculture has disrupted this cycle, leading to groundwater pollution and greenhouse gas emissions. In this study, we have demonstrated the inhibitory effects of plant-derived juglone and related 1,4-naphthoquinones on the nitrification process in Nitrosomonas europaea. Notably, the inhibition mechanism is elucidated in which 1,4-naphthoquinones interact with hydroxylamine oxidoreductase, disrupting the electron transfer to cytochrome c(554), a physiological electron acceptor. These findings support the notion that phytochemicals can impede nitrification by interfering with the essential electron transfer process in ammonia oxidation. The findings presented in this article offer valuable insights for the development of strategies aimed at the management of nitrification, reduction of fertilizer utilization, and mitigation of greenhouse gas emissions. Juglone, a plant-derived 1,4-naphthoquinone, binds to hydroxylamine oxidoreductase and inhibits the electron transfer to cytochrome c(554).,Akutsu Y, Fujiwara T, Suzuki R, Nishigaya Y, Yamazaki T Appl Environ Microbiol. 2023 Dec 21;89(12):e0129123. doi: 10.1128/aem.01291-23. , Epub 2023 Nov 27. PMID:38009977 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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