9mkl | pdb_00009mkl

canavanyl-tRNAArg deacylase (CtdA)

spinqualitylabelsall models 9mkl, resolution 1.98Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9mkl is a 8 chain structure with sequence from Salmonella enterica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.98Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0W4F7L0_SALER Publication Abstract from PubMed Aminoacyl-tRNA deacylases safeguard the accurate translation of the genetic code by hydrolyzing incorrectly synthesized aminoacyl-tRNAs. Canavanyl-tRNA deacylase (CtdA) was recently shown to protect cells against the toxicity of canavanine (Can), a nonproteinogenic amino acid synthesized and accumulated by leguminous plants. In most organisms, Can is ligated to tRNAArg, causing translation of arginine codons with Can. CtdA prevents Can toxicity by hydrolyzing canavanyl-tRNAArg. Here, we investigated the function, structure, substrate specificity, phylogenetic distribution, and evolution of CtdA. We show that CtdA is essential for preventing Can cytotoxicity in Salmonella enterica, and its heterologous expression can also protect Escherichia coli. By determining the structure of CtdA, we identified its putative binding pocket and residues that modulate enzymatic activity and specificity. We also found that CtdA displays robust specificity for the canavanyl moiety, a feature that contributes to maintaining arginyl-tRNAArg levels unaffected. Finally, we showed that despite their structural homology, CtdA and the aminoacyl-tRNA hydrolytic domain of phenylalanyl-tRNA synthetase are functionally and evolutionarily divergent. Collectively, these results substantially expand our understanding of the CtdA family, providing new insights into its structure, function, and evolution. This work also highlights the diverse mechanisms, unique to each organism, that ensure faithful translation of the genetic code. Mechanistic and evolutionary insights into a family of aminoacyl-tRNA deacylases that protects against canavanine toxicity.,Maldonado JS, Sepulveda S, Karthikeyan S, Shirakawa KT, Merced I, Radecki AA, Douglas J, Peti W, Page R, Vargas-Rodriguez O Nucleic Acids Res. 2025 Sep 5;53(17):gkaf922. doi: 10.1093/nar/gkaf922. PMID:40973455 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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