Structural highlights
Function
PMT4_YEAST Protein O-mannosyltransferase involved in O-glycosylation which is essential for cell wall rigidity. Forms a homodimeric complex to transfer mannose from Dol-P-mannose to Ser or Thr residues on proteins. Specifically acts on secretory proteins with an ER-luminally oriented Ser/Thr-rich region flanked by a membrane anchor such as FUS1, AXL2, GAS1, KEX2, MID2, WSC1, WSC2, OPY2, PRM5, RAX2, or YNL176.[1] [2] [3] [4]
References
- ↑ Sanders SL, Gentzsch M, Tanner W, Herskowitz I. O-Glycosylation of Axl2/Bud10p by Pmt4p is required for its stability, localization, and function in daughter cells. J Cell Biol. 1999 Jun 14;145(6):1177-88. PMID:10366591 doi:10.1083/jcb.145.6.1177
- ↑ Lommel M, Bagnat M, Strahl S. Aberrant processing of the WSC family and Mid2p cell surface sensors results in cell death of Saccharomyces cerevisiae O-mannosylation mutants. Mol Cell Biol. 2004 Jan;24(1):46-57. PMID:14673142 doi:10.1128/MCB.24.1.46-57.2004
- ↑ Hutzler J, Schmid M, Bernard T, Henrissat B, Strahl S. Membrane association is a determinant for substrate recognition by PMT4 protein O-mannosyltransferases. Proc Natl Acad Sci U S A. 2007 May 8;104(19):7827-32. PMID:17470820 doi:10.1073/pnas.0700374104
- ↑ Gentzsch M, Tanner W. Protein-O-glycosylation in yeast: protein-specific mannosyltransferases. Glycobiology. 1997 Jun;7(4):481-6. PMID:9184828 doi:10.1093/glycob/7.4.481
