9kua | pdb_00009kua
Crystal structure of GH5_22 exo-beta-xylosidase from the seaweed-derived thermophile Geobacillus thermodenitrificans OS27
Structural highlights
Publication Abstract from PubMedGeobacillus thermodenitrificans OS27 is a seaweed-derived thermophile that harbors a GH5_22 gene (bxlA) that encodes for a glycoside hydrolase fused with the cyclin box domain. In this study, we characterized the catalytic activity, enzymatic properties, three-dimensional structure, and physiological role of the gene product, GtBxlA. The enzyme was produced as a thermostable dimer, acting on p-nitrophenyl-beta-d-xylopyranoside among 23 substrates. beta-1,4-Linked xylooligosaccharides were also hydrolyzed from the nonreducing end. The activity indicated that GtBxlA functions as an exo-beta-1,4-xylosidase. We determined the crystal structure of the Glu188Ala variant complexed with beta-1,4-xylotriose at 1.52 A resolution. GtBxlA exhibited an atypical (beta/alpha)(8)-barrel architecture. The cyclin box constituted a single alpha-helix within the core barrel and a lid-like domain that contributes to dimer formation. Structural analysis revealed that Glu188 and Glu318 are positioned to serve as the acid/base and nucleophile catalysts, respectively. Alanine mutagenesis confirmed the essential role of Glu188 and Glu318 in catalysis. We also determined the ligand-free structure of the Glu188Ala variant. Both structures were almost identical; however, a loop at the substrate entry site was fixed in the ligand-free structure, suggesting a conformational change upon substrate binding. Although bxlA deletion did not affect beta-1,4-xylan utilization, its expression was induced by beta-1,4-xylan. These observations suggest that G. thermodenitrificans OS27 employed GtBxlA in utilizing beta-1,4-xylan. Notably, GtBxlA could hydrolyze beta-1,3-linked xylooligosaccharides. This highlights the possibility that GtBxlA also assists the host in utilizing beta-1,3-xylan, which is abundant in certain seaweeds. Structural and biochemical characterization of a GH5_22 enzyme from the seaweed-derived thermophile Geobacillus thermodenitrificans OS27.,Lee W, Hino T, Fujii K, Tanimoto S, Naka R, Hara K, Okamoto T, Okazaki F, Nagano S, Ohshiro T, Suzuki H Arch Biochem Biophys. 2026 Jan 16;778:110736. doi: 10.1016/j.abb.2026.110736. PMID:41548701[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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