9m21 | pdb_00009m21

GmMAN19-1 from Glycine max in complex with mannopentaose

spinqualitylabelsall models 9m21, resolution 2.62Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9m21 is a 1 chain structure with sequence from Glycine max. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.621Å Ligands: BMA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Endo-1,4-beta-mannanase (EC 3.2.1.78) cleaves beta-1,4-mannans in cell walls, facilitating endosperm softening and seed germination. Here, we present the structural and biochemical characterization of GmMAN19-1, a GH5_7 beta-mannanase from soybean (Glycine max), a crop with substantial agricultural importance. GmMAN19-1 is specifically expressed in cotyledons during postgermination and exhibits acidophilic activity optimal at pH 4.6 and 40 degrees C. Crystal structures of GmMAN19-1 were determined at 1.39 and 2.62 A in the apo form and in complex with mannopentaose (M5), respectively. The structure adopts a canonical (alpha/beta)(8) TIM barrel fold with a V-shaped active site groove. Notably, two distinct M5 binding modes were identified, suggesting dual functionality involving hydrolytic and transglycosylation activities. Site-directed mutagenesis further validated key catalytic and substrate-interacting residues: E186A abolished enzymatic activity, while Q267W altered transglycosylation product profiles and enhanced activity toward branched substrates. The binding groove can accommodate galactose side chains, supporting partial activity toward galactomannans. These findings provide comprehensive insights into the substrate specificity and catalytic mechanism of plant beta-mannanases and establish GmMAN19-1 as a potential candidate for applications in food processing, biomass conversion, and industrial biotechnology, particularly due to its acidophilic nature and enhanced activity toward branched mannans. Unveiling the Structural and Biochemical Characteristics of an Acidophilic beta-Mannanase from Soybean (Glycine max).,Lin CJ, Cho CC, Chen SC, Lin GM, Huang CY, Hsu CH J Agric Food Chem. 2025 Oct 8;73(40):25479-25490. doi: 10.1021/acs.jafc.5c03141. , Epub 2025 Sep 24. PMID:40994130 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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