4wy9 | pdb_00004wy9

Crystal structure of the periplasmic sensory domain of the Campylobacter jejuni chemoreceptor Tlp1

spinqualitylabelsall models 4wy9, resolution 1.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4wy9 is a 1 chain structure with sequence from Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.4Å Ligands: ACT, CL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q0P8B2_CAMJE Publication Abstract from PubMed Chemotaxis and motility play an important role in the colonisation of avian and human hosts by Campylobacter jejuni. Chemotactic recognition of extracellular signals is mediated by the periplasmic sensing domain of methyl-accepting chemotactic proteins (membrane-embedded receptors). In this work, we report a high-resolution structure of the periplasmic sensing domain of transducer-like protein 1 (Tlp1), an aspartate receptor of C. jejuni. Crystallographic analysis revealed that it contains two Per-Arnt-Sim (PAS) subdomains. An acetate and chloride ions (both from the crystallisation buffer) were observed bound to the membrane-proximal and membrane-distal PAS subdomains, respectively. Surprisingly, despite being crystallised in the presence of aspartate, the structure did not show any electron density corresponding to this amino acid. Furthermore, no binding between the sensing domain of Tlp1 and aspartate was detected by microcalorimetric experiments. These structural and biophysical data suggest that Tlp1 does not sense aspartate directly; instead, ligand recognition is likely to occur indirectly via an as yet unidentified periplasmic binding protein. The Crystal Structure of the Tandem-PAS Sensing Domain of Campylobacter jejuni Chemoreceptor Tlp1 Suggests Indirect Mechanism of Ligand Recognition.,Machuca MA, Liu YC, Beckham SA, Gunzburg MJ, Roujeinikova A J Struct Biol. 2016 Feb 23. pii: S1047-8477(16)30034-X. doi:, 10.1016/j.jsb.2016.02.019. PMID:26923153 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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