7ugs | pdb_00007ugs

Crystal structure of monomeric hyperfolder YFP (K206V mutant)

spinqualitylabelsall models 7ugs, resolution 1.63Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7ugs is a 1 chain structure with sequence from Aequorea victoria. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.63Å Ligands: CR2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A059PIR9_AEQVI Publication Abstract from PubMed We report the rational engineering of a remarkably stable yellow fluorescent protein (YFP), 'hyperfolder YFP' (hfYFP), that withstands chaotropic conditions that denature most biological structures within seconds, including superfolder green fluorescent protein (GFP). hfYFP contains no cysteines, is chloride insensitive and tolerates aldehyde and osmium tetroxide fixation better than common fluorescent proteins, enabling its use in expansion and electron microscopies. We solved crystal structures of hfYFP (to 1.7-A resolution), a monomeric variant, monomeric hyperfolder YFP (1.6 A) and an mGreenLantern mutant (1.2 A), and then rationally engineered highly stable 405-nm-excitable GFPs, large Stokes shift (LSS) monomeric GFP (LSSmGFP) and LSSA12 from these structures. Lastly, we directly exploited the chemical stability of hfYFP and LSSmGFP by devising a fluorescence-assisted protein purification strategy enabling all steps of denaturing affinity chromatography to be visualized using ultraviolet or blue light. hfYFP and LSSmGFP represent a new generation of robustly stable fluorescent proteins developed for advanced biotechnological applications. Chemically stable fluorescent proteins for advanced microscopy.,Campbell BC, Paez-Segala MG, Looger LL, Petsko GA, Liu CF Nat Methods. 2022 Dec;19(12):1612-1621. doi: 10.1038/s41592-022-01660-7. Epub , 2022 Nov 7. PMID:36344833 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Green Fluorescent Protein 3D structures

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