| Structural highlights
8ztb is a 2 chain structure with sequence from Serratia marcescens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.9Å |
| Ligands: | ACT, CL, NA, PEG, PO4, SO4, ZN |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
BLBI1_SERMA Class B beta-lactamase which confers resistance to the beta-lactam antibiotics, including penicillins, cephalosporins and carbapenems (PubMed:10898670, PubMed:29485857, PubMed:8141584). Acts via hydrolysis of the beta-lactam ring (PubMed:10898670, PubMed:15788415). Has penicillin-, cephalosporin- and carbapenem-hydrolyzing activities (PubMed:10898670, PubMed:15788415, PubMed:15892033). Has endoribonuclease activity, cleaving substrate RNAs preferentially between U/C and A, in vitro (PubMed:33126240).[1] [2] [3] [4] [5] [6]
References
- ↑ Iyobe S, Kusadokoro H, Ozaki J, Matsumura N, Minami S, Haruta S, Sawai T, O'Hara K. Amino acid substitutions in a variant of IMP-1 metallo-beta-lactamase. Antimicrob Agents Chemother. 2000 Aug;44(8):2023-7. PMID:10898670 doi:10.1128/AAC.44.8.2023-2027.2000
- ↑ Yamaguchi Y, Kuroki T, Yasuzawa H, Higashi T, Jin W, Kawanami A, Yamagata Y, Arakawa Y, Goto M, Kurosaki H. Probing the role of Asp-120(81) of metallo-beta-lactamase (IMP-1) by site-directed mutagenesis, kinetic studies, and X-ray crystallography. J Biol Chem. 2005 May 27;280(21):20824-32. Epub 2005 Mar 23. PMID:15788415 doi:10.1074/jbc.M414314200
- ↑ Kurosaki H, Yamaguchi Y, Higashi T, Soga K, Matsueda S, Yumoto H, Misumi S, Yamagata Y, Arakawa Y, Goto M. Irreversible inhibition of metallo-beta-lactamase (IMP-1) by 3-(3-mercaptopropionylsulfanyl)propionic acid pentafluorophenyl ester. Angew Chem Int Ed Engl. 2005 Jun 20;44(25):3861-4. PMID:15892033 doi:10.1002/anie.200500835
- ↑ Hinchliffe P, Tanner CA, Krismanich AP, Labbe G, Goodfellow VJ, Marrone L, Desoky AY, Calvopina K, Whittle EE, Zeng F, Avison MB, Bols NC, Siemann S, Spencer J, Dmitrienko GI. Structural and Kinetic Studies of the Potent Inhibition of Metallo-beta-lactamases by 6-Phosphonomethylpyridine-2-carboxylates. Biochemistry. 2018 Mar 27;57(12):1880-1892. doi: 10.1021/acs.biochem.7b01299., Epub 2018 Mar 9. PMID:29485857 doi:https://dx.doi.org/10.1021/acs.biochem.7b01299
- ↑ Kato Y, Takahashi M, Seki M, Nashimoto M, Shimizu-Ibuka A. RNA-hydrolyzing activity of metallo-β-lactamase IMP-1. PLoS One. 2020 Oct 30;15(10):e0241557. PMID:33126240 doi:10.1371/journal.pone.0241557
- ↑ Osano E, Arakawa Y, Wacharotayankun R, Ohta M, Horii T, Ito H, Yoshimura F, Kato N. Molecular characterization of an enterobacterial metallo beta-lactamase found in a clinical isolate of Serratia marcescens that shows imipenem resistance. Antimicrob Agents Chemother. 1994 Jan;38(1):71-8. PMID:8141584 doi:10.1128/AAC.38.1.71
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