Structural highlights
Publication Abstract from PubMedEukaryotic ribosomes are enriched with pseudouridine, particularly at the functional centers targeted by antibiotics. Here, we investigated the roles of pseudouridine in aminoglycoside-mediated translation inhibition by comparing the structural and functional properties of the yeast wild-type and the pseudouridine-free ribosomes. We showed that the pseudouridine-free ribosomes have decreased thermostability and high sensitivity to aminoglycosides. When presented with a model internal ribosomal entry site RNA, elongation factor eEF2, GTP (guanosine triphosphate), and sordarin, hygromycin B preferentially binds to the pseudouridine-free ribosomes during initiation by blocking eEF2 binding, stalling ribosomes in a nonrotated conformation. The structures captured hygromycin B bound at the intersubunit bridge B2a enriched with pseudouridine and a deformed codon-anticodon duplex, revealing a functional link between pseudouridine and aminoglycoside inhibition. Our results suggest that pseudouridine enhances both thermostability and conformational fitness of the ribosomes, thereby influencing their susceptibility to aminoglycosides. Structural basis for hygromycin B inhibition of yeast pseudouridine-deficient ribosomes.,Zhao Y, Xu C, Chen X, Jin H, Li H Sci Adv. 2025 Apr 4;11(14):eadu0151. doi: 10.1126/sciadv.adu0151. Epub 2025 Apr , 2. PMID:40173234[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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