Structural highlights
FunctionDNAT_ECOLI This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with DnaB protein and other prepriming proteins DnaC, N, N', N a prepriming protein complex on the specific site of the template DNA recognized by protein N'.[HAMAP-Rule:MF_01061] Publication Abstract from PubMedReplication restart pathways reinitiate DNA replication processes following their premature termination. In Escherichia coli, this essential process begins with regulated assembly of the preprimosome complex, comprising the PriA, PriB, and DnaT proteins, onto an abandoned replication fork. Here, we present two distinct preprimosome structures. One represents an intermediate stage in preprimosome assembly with a single DnaT C-terminal domain (DnaT(CTD)) bound to PriA/PriB/DNA. The second captures the mature preprimosome, in which filamentation of multiple DnaT(CTD) molecules catalyzes the handoff of the single-stranded lagging-strand DNA from PriB to DnaT. The DnaT N-terminal domain forms a separate, independent oligomer in the mature structure. Taken together, our results detail the molecular mechanisms underlying replication restart initiation and regulation and suggest mechanistic similarities between DnaT and the canonical initiator protein DnaA. Visualization of the complete preprimosome reveals the structural mechanisms governing DNA replication restart.,Ducos PL, Duckworth AT, Satyshur KA, Keck JL, Grant T Nat Commun. 2026 May 16. doi: 10.1038/s41467-026-73239-1. PMID:42140935[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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