30gj | pdb_000030gj

W-formate dehydrogenase from Nitratidesulfovibrio vulgaris (Desulfovibrio vulgaris) - Ambient temperature, Serial crystallography

spinqualitylabelsall models 30gj, resolution 1.93Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 30gj is a 2 chain structure with sequence from Desulfovibrio vulgaris str. Hildenborough. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.93Å Ligands: H2S, MGD, W Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q72EJ1_NITV2 Publication Abstract from PubMed Metal-dependent formate dehydrogenases (Fdhs) are a promising research target in efforts to mitigate climate change by developing active, efficient, selective and safe industrial catalysts for CO(2) reduction. Here, we report the room-temperature (RT) serial synchrotron X-ray crystallography (SSX) structure of Nitratidesulfovibrio vulgaris formate dehydrogenase AB and compare this structure with previously reported cryogenic structures. The comparison reveals structural differences likely arising from the absence of cryoprotectants, differences in data-collection temperature and the much lower radiation dose accumulated by each crystal during the SSX experiments. The RT-SSX structure provides the first step towards time-resolved serial crystallography experiments targeting possible catalytic intermediates in order to fully understand the catalytic mechanism of metal-dependent Fdhs. Room-temperature crystal structure of a metal-dependent W-formate dehydrogenase by serial synchrotron crystallography.,Vilela-Alves G, Martins G, von Stetten D, Mehrabi P, Pereira IAC, Romao MJ, Pearson AR, Mota C Acta Crystallogr D Struct Biol. 2026 Sep 1. doi: 10.1107/S2059798326006923. PMID:42572941 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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