23vw | pdb_000023vw

Cryo-EM structure of the TfCas12m3-crRNA-tgDNA ternary complex

spinqualitylabelsall models 23vw, resolution 2.39Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 23vw is a 4 chain structure with sequence from DNA molecule and Peptococcaceae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.39Å Ligands: MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CS12M_THEFR CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids) (Probable) (PubMed:38261981). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids (Probable) (PubMed:38261981). CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (PubMed:38261981). Recognizes a short motif in the CRISPR repeat sequences (the 5' PAM or protospacer adjacent motif, 5'-C/TCN-3' in this organism) to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs (PubMed:38261981). Upon expression in E.coli as a CRISPR locus inhibits plasmid propagation when targeted to regions essential for plasmid propagation (replication origin but not a selectable marker), probably by inhibiting transcription (PubMed:38261981). Cas12m-crRNA binds DNA in a PAM-dependent, crRNA-guided fashion (PubMed:38261981). Upon expression in E.coli as a CRISPR region preferentially binds to its associated crRNA (PubMed:38261981). Probably required for pre-crRNA processing to mature crRNA (Probable) (PubMed:38261981).

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