2dyb | pdb_00002dyb
The crystal structure of human p40(phox)
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Overview
The superoxide-producing phagocyte NADPH oxidase is activated during, phagocytosis to destroy ingested microbes. The adaptor protein p40(phox), associates via the PB1 domain with the essential oxidase activator, p67(phox), and is considered to function by recruiting p67(phox) to, phagosomes; in this process, the PX domain of p40(phox) binds to, phosphatidylinositol 3-phosphate [PtdIns(3)P], a lipid abundant in the, phagosomal membrane. Here we show that the PtdIns(3)P-binding activity of, p40(phox) is normally inhibited by the PB1 domain both in vivo and in, vitro. The crystal structure of the full-length p40(phox) reveals that the, inhibition is mediated via intramolecular interaction between the PB1 and, PX domains. The interface of the p40(phox) PB1 domain for the PX domain, localizes on the opposite side of that for the p67(phox) PB1 domain, and, thus the PB1-mediated PX regulation occurs without preventing the PB1-PB1, association with p67(phox).
About this Structure
2DYB is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Full-length p40(phox) structure suggests a basis for regulation mechanism of its membrane binding., Honbou K, Minakami R, Yuzawa S, Takeya R, Suzuki NN, Kamakura S, Sumimoto H, Inagaki F, EMBO J. 2007 Feb 21;26(4):1176-86. Epub 2007 Feb 8. PMID:17290225
Page seeded by OCA on Mon Nov 12 21:40:50 2007
