1efa | pdb_00001efa
CRYSTAL STRUCTURE OF THE LAC REPRESSOR DIMER BOUND TO OPERATOR AND THE ANTI-INDUCER ONPF
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Overview
Crystal structures of the Lac repressor, with and without, isopropyithiogalactoside (IPTG), and the repressor bound to operator have, provided a model for how the binding of the inducer reduces the affinity, of the repressor for the operator. However, because of the low resolution, of the operator-bound structure (4.8 A), the model for the allosteric, transition was presented in terms of structural elements rather than in, terms of side chain interactions. Here we have constructed a dimeric Lac, repressor and determined its structure at 2.6 A resolution in complex with, a symmetric operator and the anti-inducer orthonitrophenylfucoside (ONPF)., The structure enables the induced (IPTG-bound) and repressed, (operator-bound) conformations of the repressor to be compared in atomic, detail. An extensive network of interactions between the DNA-binding and, core domains of the repressor suggests a possible mechanism for the, allosteric transition.
About this Structure
1EFA is a Single protein structure of sequence from Escherichia coli with NPF as ligand. The following page contains interesting information on the relation of 1EFA with [lac Repressor]. Full crystallographic information is available from OCA.
Reference
A closer view of the conformation of the Lac repressor bound to operator., Bell CE, Lewis M, Nat Struct Biol. 2000 Mar;7(3):209-14. PMID:10700279
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