1akg | pdb_00001akg
ALPHA-CONOTOXIN PNIB FROM CONUS PENNACEUS
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Overview
Two mollusc-specific neurotoxic peptides from the venom of the, molluscivorous snail Conus pennaceus are described. These new toxins block, acetylcholine receptors (AChR) of cultured Aplysia neurons. Bath, application of 0.5-1 microM toxin induces 5-10-mV membrane depolarization, which recovers to the control level within 1-3 min in the presence of the, toxin. This response is blocked by 1 mM hexamethonium. Concomitantly with, the transient depolarization, the toxins block approximately 90% of the, depolarizing responses evoked by brief iontophoretic application of, acetylcholine. The pharmacology and amino acid sequences of the toxins, (alpha PnIA, GCCSLPPCAANNPDYC-NH2; alpha PnIB, GCCSLPPCALSNPDYC-NH2), enable their classification as novel alpha-conotoxins. The sequences, differ from those of previously described alpha-conotoxins in a number of, features, the most striking of which is the presence of a single, negatively charged residue in the C-terminal loop. This loop contains a, positively charged residue in piscivorous venom alpha-conotoxins. In, contrast to other alpha-conotoxins, which are selective for vertebrate, skeletal muscle nicotinic ACh receptors, these Conus pennaceus toxins, block neuronal ACh receptors in molluscs. As such they are new probes, which can be used to define subtypes of ACh receptors, and they should be, useful tools in the study of structure-function relationships in ACh, receptors.
About this Structure
1AKG is a Single protein structure of sequence from Conus pennaceus with NH2 as ligand. Full crystallographic information is available from OCA.
Reference
New mollusc-specific alpha-conotoxins block Aplysia neuronal acetylcholine receptors., Fainzilber M, Hasson A, Oren R, Burlingame AL, Gordon D, Spira ME, Zlotkin E, Biochemistry. 1994 Aug 16;33(32):9523-9. PMID:8068627
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