1dod | pdb_00001dod
THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS
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Overview
Para-hydroxybenzoate hydroxylase inserts oxygen into substrates by means, of the labile intermediate, flavin C(4a)-hydroperoxide. This reaction, requires transient isolation of the flavin and substrate from the bulk, solvent. Previous crystal structures have revealed the position of the, substrate para-hydroxybenzoate during oxygenation but not how it enters, the active site. In this study, enzyme structures with the flavin ring, displaced relative to the protein were determined, and it was established, that these or similar flavin conformations also occur in solution., Movement of the flavin appears to be essential for the translocation of, substrates and products into the solvent-shielded active site during, catalysis.
About this Structure
1DOD is a Single protein structure of sequence from Pseudomonas aeruginosa with FAD and DOB as ligands. Full crystallographic information is available from OCA.
Reference
The mobile flavin of 4-OH benzoate hydroxylase., Gatti DL, Palfey BA, Lah MS, Entsch B, Massey V, Ballou DP, Ludwig ML, Science. 1994 Oct 7;266(5182):110-4. PMID:7939628
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