1nco | pdb_00001nco
STRUCTURE OF THE ANTITUMOR PROTEIN-CHROMOPHORE COMPLEX NEOCARZINOSTATIN
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Overview
Structures of the protein-chromophore complex and the apoprotein form of, neocarzinostatin were determined at 1.8 angstrom resolution., Neocarzinostatin is composed of a labile chromophore with DNA-cleaving, activity and a stabilizing protein. The chromophore displays marked, nonlinearity of the triple bonds and is bound noncovalently in a pocket, formed by the two protein domains. The chromophore pi-face interacts with, the phenyl ring edges of Phe52 and Phe78. The amino sugar and carbonate, groups of the chromophore are solvent exposed, whereas the epoxide, acetylene groups, and carbon C-12, the site of nucleophilic thiol addition, during chromophore activation, are unexposed. The position of the amino, group of the chromophore carbohydrate relative to C-12 supports the idea, that the amino group plays a role in thiol activation.
About this Structure
1NCO is a Single protein structure of sequence from Streptomyces carzinostaticus with CHR and MRD as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of neocarzinostatin, an antitumor protein-chromophore complex., Kim KH, Kwon BM, Myers AG, Rees DC, Science. 1993 Nov 12;262(5136):1042-6. PMID:8235619
Page seeded by OCA on Tue Nov 20 22:08:26 2007
