1pp5 | pdb_00001pp5
Structure of Antibacterial Peptide Microcin J25: a 21-Residue Lariat Protoknot
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Overview
The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue "lariat protoknot", consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.
About this Structure
1PP5 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of antibacterial peptide microcin J25: a 21-residue lariat protoknot., Bayro MJ, Mukhopadhyay J, Swapna GV, Huang JY, Ma LC, Sineva E, Dawson PE, Montelione GT, Ebright RH, J Am Chem Soc. 2003 Oct 15;125(41):12382-3. PMID:14531661
Page seeded by OCA on Tue Nov 20 23:59:25 2007
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Escherichia coli
- Single protein
- Bayro, M.J.
- Ebright, R.H.
- Huang, J.Y.
- Ma, L.C.
- Montelione, G.T.
- Mukhopadhyay, J.
- NESG, Northeast.Structural.Genomics.Consortium.
- Swapna, G.V.T.
- Backbone-sidechain amide linkage
- Lariat
- Nesg
- Northeast structural genomics consortium
- Protein structure initiative
- Protoknot
- Psi
- Structural genomics
