1qvc | pdb_00001qvc
CRYSTAL STRUCTURE ANALYSIS OF SINGLE STRANDED DNA BINDING PROTEIN (SSB) FROM E.COLI
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Overview
The single-stranded DNA (ssDNA) binding protein from Escherichia coli, (EcoSSB) plays a central role in DNA replication, recombination and, repair. The tertiary structure of EcoSSB was determined at 2.2 A, resolution. This is rather higher resolution than previously reported., Crystals were grown from the homogeneous intact protein but the EcoSSB, tetramer in the crystals contains truncated subunits lacking a part of the, C-terminal. The structure determined includes biologically important, flexible loops and C-terminal regions, and revealed the existence of, concavities. These concavities include the residues important for ssDNA, binding. An ssDNA can be fitted on the concavities and further stabilized, through interactions with the loops forming flexible lids. It seems likely, to play a central role in the binding of ssDNA.
About this Structure
1QVC is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Roles of functional loops and the C-terminal segment of a single-stranded DNA binding protein elucidated by X-Ray structure analysis., Matsumoto T, Morimoto Y, Shibata N, Kinebuchi T, Shimamoto N, Tsukihara T, Yasuoka N, J Biochem (Tokyo). 2000 Feb;127(2):329-35. PMID:10731701
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