1ryk | pdb_00001ryk

Solution NMR Structure Protein yjbJ from Escherichia coli. Northeast Structural Genomics Consortium Target ET93; Ontario Centre for Structural Proteomics target EC0298_1_69;

File:1ryk.gif

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Overview

The influx of genomic sequence information has led to the concept of, structural proteomics, the determination of protein structures on a, genome-wide scale. Here we describe an approach to structural proteomics, of small proteins using NMR spectroscopy. Over 500 small proteins from, several organisms were cloned, expressed, purified, and evaluated by NMR., Although there was variability among proteomes, overall 20% of these, proteins were found to be readily amenable to NMR structure determination., NMR sample preparation was centralized in one facility, and a distributive, approach was used for NMR data collection and analysis. Twelve structures, are reported here as part of this approach, which allowed us to infer, putative functions for several conserved hypothetical proteins.

About this Structure

1RYK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

An NMR approach to structural proteomics., Yee A, Chang X, Pineda-Lucena A, Wu B, Semesi A, Le B, Ramelot T, Lee GM, Bhattacharyya S, Gutierrez P, Denisov A, Lee CH, Cort JR, Kozlov G, Liao J, Finak G, Chen L, Wishart D, Lee W, McIntosh LP, Gehring K, Kennedy MA, Edwards AM, Arrowsmith CH, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1825-30. PMID:11854485

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